摘要
Abstract
Objective To explore the immunological machamism of interleukin-17(IL-17) and intercellular adhesion molecu-lar-l(ICAM-l) in the progress of Systemic Lupus Erythematosus(SLE). Methods According to valentijn method to determine the standard reference to collect 46 SLE patients in Longgang Central Hospital from May 2008 to August 2011, then classfied the activities of the 29 cases and 17 cases of remission. Venous blood was taken early in the morning and peripheral blood mononuclear cell culture supernatants was prepared. The sera of SLE patients and PMBC supernatant IL-17 and ICAM-1 was tesed by ELISA,at the same time the 42 healthy controls was taken. The parameters of IL-17 and ICAM-1 was compared with the commonly used laboratory parameters ANA.anti ds-DNA indicators. Results IL-17 and ICAM-1 levels in the serum of patients with SLE were 59. 12±5. 69 μmol/L and 494. 5±86. 51μg/L) significantly higher than SLE remission group of serum IL-17 and ICAM-1 levels 27. 13 ±3. 05 μmol/L and 185. 80 ± 32. 11 μg/L and normal control serum IL-17 and ICAM-1 levels 9. 50±1. 24 μmol/L and 91. 73±30. 10 μg/L, t value of 3. 859, P value 0. 021. Similarly,patients with SLEPBMC supernatant IL-17 and ICAM-1 levels 187. 76 ±27. 10 μmol/L and 582.20 ± 91.87 fig/L, significantly higher than SLE remission group PBMC supernatant IL-17 and ICAM-1 levels 45. 83 ±3. 97 pmol/L and 191. 73 ±30. 10 μg/L) and normal control group PBMC supernatant IL-17 and ICAM-1 levels 12. 64±2. 52 pmol/L and 214. 48±40. 07 μg/ L, t value 4. 051, P-value of 0. 028. And serum IL-17 and ICAM-1 levels 68. 36±6. 09 μmol/L and 600. 24 ± 91. 61 μg/L, t-value of 5. 909, P-value of 0. 024 in the ANA,antids-DNA-positive patients with SLE were Significantly higher than the serum IL-17 and ICAM-1 levels 24. 05±3. 17 μmol/L and 289. 55 ± 34. 81 μg/L, t value 2. 790, P value of 0. 038 in the ANA, anti ds-DNA negative SLE. And IL-17 and ICAM-1 were deceased significantly after effective therapy. Conclusion IL-17 and ICAM-1 may be one of the immunological function disorders, at the same time, the quantity detection of IL-17 and ICAM-1 is benefit to predict the progress and outcome of SLE. The increase of serum ICAM-1 levels during the active period of SLE d may determine the reference index.关键词
系统性红斑狼疮/白细胞介素17/细胞间黏附分子-1Key words
systemic lupus erythematosus/interleukin-17/ intercellular adhesion molecular-1分类
医药卫生
