石油勘探与开发2012，Vol.39Issue(4)：505-512,8.
利用分子生物学技术分析生物气藏中甲烷形成途径
Methane production pathway in biogas reservoirs via molecular biological technique
摘要
Abstract
To investigate the microbes in formation water samples from Well Du 6-3 and Ao-7 in the Aiaxin and Aonan gas fields, Songliao Basin, 16S rDNA was amplified using the Polymerase Chain Reaction (PCR) technique with templates from the extracted DNA and primers of bacteria and arehaea. The PCR products were also inserted into vectors and then ligated with competent cells for gene sequencing. Based on the obtained gene sequences, bacterial and archaeal libraries were constructed and microbiological phytogeny graphs were obtained for the samples to understand the structure of microbial community and deduce the possible pathways of methanogenesis. The result indicates that there are only a few types of microbes in the samples of the two wells. Pseudomonas and Methanobacterium are the dominant species in the microbial and archaeal libraries, respectively. Methane generation via indigenous microbial metabolism may be ultimately realized using the right method under favourable conditions in the two fields. It is concluded that the indigenous microbial community in Well Du 6-3 mainly generated methane by CO2 reduction, but methanogenesis of acetic acid could not be completely excluded. The methane from Well Ao-7 is shown to have been definitely converted from CQ2.关键词
生物气藏/聚合酶切链式反应/甲烷生物合成途径/16S rRNA克隆文库Key words
biogas reservoir/ polymerase chain reaction (PCR)/ methane bio-pathway/ 16S rRNA clone library分类
能源科技引用本文复制引用
魏小芳,秦积舜,帅燕华,刘可禹,罗一菁,史彦尧,张曙光..利用分子生物学技术分析生物气藏中甲烷形成途径[J].石油勘探与开发,2012,39(4):505-512,8.基金项目
国家自然科学基金(NSF40873031) （NSF40873031）
中石油国际合作项目(2011A-1004-01) （2011A-1004-01）
中石油创新基金(2009D-5006-01-01) （2009D-5006-01-01）
