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大肠癌组织库建立及质量控制的经验

王聪 张大庆 赵盈盈 仇长青 杜勇 李海 翟惠虹

实用肿瘤杂志2012,Vol.27Issue(4):406-410,5.
实用肿瘤杂志2012,Vol.27Issue(4):406-410,5.

大肠癌组织库建立及质量控制的经验

Establishment of colorectal cancer tissue bank and quality control

王聪 1张大庆 1赵盈盈 1仇长青 1杜勇 2李海 3翟惠虹4

作者信息

  • 1. 宁夏医科大学临床医学院,宁夏银川750004
  • 2. 宁夏医科大学附属总医院外科实验室,宁夏银川750004
  • 3. 宁夏医科大学附属总医院结直肠外科,宁夏银川750004
  • 4. 宁夏医科大学附属总医院消化内科,宁夏银川750004
  • 折叠

摘要

Abstract

Objective To establish a standardized colorectal cancer tissue bank. Methods The project and protocols were approved by Ethical Committee of the hospital. Colorectal cancer tissues were collected from patients and stored in freezer. Results A standard protocol for sample collection was developed. During one year period 587 samples of colorectal cancer tissue and 549 serum samples were stored. RNA and the genomic DNA of the randomly selected specimens were of good quality. The percentages of cancer cells in cancer samples were greater than 80% and the specimens collected were associated with complete clinical data. Conclusion The colorectal cancer tissue bank established in this study can fully meet the requirements of clinical and basic research.

关键词

结直肠肿瘤/病理学/组织库/生物标本库/标准/质量控制

Key words

colorectal neoplasms/pathology/ tissue banks/ biological specimen banks/standards/ quality control

分类

医药卫生

引用本文复制引用

王聪,张大庆,赵盈盈,仇长青,杜勇,李海,翟惠虹..大肠癌组织库建立及质量控制的经验[J].实用肿瘤杂志,2012,27(4):406-410,5.

基金项目

宁夏高等学校科学技术研究项目 ()

实用肿瘤杂志

OACSTPCD

1001-1692

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