生殖医学杂志2012，Vol.21Issue(4)：368-375,8.DOI:10.3969/j.issn.1004-3845.2012.04.015
睾丸组织高表达基因PRKACG对细胞增殖的影响
The effect of a testicular highly expression gene PRKACG on cell proliferation
摘要
Abstract
Objective: To study the function of a testicular highly expression gene PRKACG (protein kinase, cAMP-dependent, catalytic, gamma), and find out novel diagnosis and therapeutic targets for the treatment of male infertility. Methods: The activation effect of PRKACG in the NF-kB signaling pathways that are related to spermatogenesis was analyzed by using dual luciferase report system, and the effect of PRKACG on cell proliferation was further studied by morphological observation, cell counting and CCK8 assay. Results: The 293T cells present the changes containing volume fill-out, full, antenna elongation, connection large expanse mostly when PRKACG transfect in the cells. Cell counting and CCK8 assays showed that PRKACG expression enhances overall cell growth rate compared with Sport 6. Conclusions; PRKACG could activate NF-kB signaling pathway, and promote cell proliferation, indicating that PRKACG might be a novel functional gene in testicular development and spermatogenesis.关键词
cAMP依赖蛋白激酶催化亚基γ/男性不育/睾丸发育/精子发生/细胞增殖Key words
PRKACG/ Male infertility/ Testicular development/ Spermatogenesis/ Cell proliferation引用本文复制引用
宋亚娟,孟雅楠,刘青,贾孟春,张秀军..睾丸组织高表达基因PRKACG对细胞增殖的影响[J].生殖医学杂志,2012,21(4):368-375,8.基金项目
国家自然科学基金(81072093,81170616) （81072093,81170616）
