天津中医药2012，Vol.29Issue(4)：349-355,7.
针刺内关穴对急性心肌缺血大鼠缺血心肌基因表达谱的影响
Effect of acupuncture at Neiguan acupoint on the gene expression profile of rats with acute myocardial ischemia
摘要
Abstract
[Objective] To study the effect of acupuncture on the gene expression profile in acute myocardial ischemia. [Methods] Aacute myocardial ischemic was induced by ligating the left anterior descending branch of coronary artery,and the total RNA of the ischemic myocardium was extracted after grouping and intervention. Afterwards,we hybridized the extraction with the whole gene expression profile chip in rat and detected them. In the differential expressed gene during therapeutic process after screening,we searched and analyzed the compound functional classification of the significant aggregative differential expression by the means of gene functional classification system and discussed the effect of acupuncture on the gene expression profile in acute myocardial ischemia from the level of gene function. [Results] The expression of 398 genes was up-regulated after 24 hours of myocardial ischemia. However,the expression of 298 gene (74.87% of the total up-regulated genes) showed the tendency of down-regulation after acupuncture at Neiguan acupoint three times intermittently; in the 338 down-regulated genes after 24 hours of myocardial ischemia,the expression of 188 genes (55.62% of the total down-regulated genes) showed the tendency of up-regulation after acupuncture at Neiguan acupoint three times intermittently,indicating a benign regulative action of the expression of ischemia myocardium gene after acupuncture at Neiguan acupoint. In the screened differential expression genes,the functional module of Ppmlb,Suclgl,Got2,Mdhl,PDP1,Fh,Cpb2,Ywhaz,S100A9,Atplbl,Tnl,Myl3,Casq2,Mb,mainly involved the oxidative phosphorylation,the electron transfer of mitochodria,the electron transfer coupling of ATP synthesis,the energy metabolism,the process of phoshates metabolism,the univalent inorganic cation transfer,the process of coenzyme metabolism and so on. [Conclusion] Acupuncture at Neiguan acupoint may protect the myocardium by influencing the genes mentioned above.关键词
针刺/急性心肌缺血/基因功能表达Key words
acupuncture/acute myocardium ischemia/expression of gene function分类
医药卫生引用本文复制引用
梁宪如,席强,李晓梅,崔瑞,金光,郭义,郭永明..针刺内关穴对急性心肌缺血大鼠缺血心肌基因表达谱的影响[J].天津中医药,2012,29(4):349-355,7.基金项目
天津市高等学校科技发展基金项目(020327). （020327）
