天然产物研究与开发2012，Vol.24Issue(4)：498-502,5.
棉叶中棉酚的快速提取与测定方法研究
Quick Extraction and Analysis of Gossypol in Cotton Leaves
摘要
Abstract
The terpenoids in cotton contain a unique group of secondary metabolites, which include desoxyhermgossypol (DHG),hemigosaypolone (HGQ) ,hemigossypol (HG) ,goasypol(G) ,and heliocides H1,H2,H3 and H4(H4). These compounds have been shown to be important in protecting the plant from cotton insects. An HPLC method has been utilized for separating individual terpenoid and quantitating gossypol in cotton leaves. Some influencing factors,such as extracting and pre-treating process,were discussed and a set of simple,rapid method was developed. At the same time,the result of the UV-visible spectmphotometry (aniline method) was described and compared. The results showed that HPLC analysis was more reasonable for the insect-resistance investigation in cotton.关键词
棉酚/HPLC/苯胺比色法Key words
gossypol/aniline method/HPLC分类
医药卫生引用本文复制引用
景伟文,遆晓南,陈燕勤,张坤驰,刘喜..棉叶中棉酚的快速提取与测定方法研究[J].天然产物研究与开发,2012,24(4):498-502,5.基金项目
国家自然科学基金项目(30760135) （30760135）
