土壤学报2012，Vol.49Issue(3)：567-574,8.
不同施肥模式对绿洲农田土壤微生物群落丰度与酶活性的影响
EFFECT OF MODEL OF FERTILIZATION ON MICROBIAL ABUNDANCE AND ENZYME ACTIVITY IN OASIS FARMLAND SOIL
摘要
Abstract
A 20-year field experiment an fertilization models, e. g. applications of inorganic fertilizer alone (CF) and inorganic fertilizer combined with wheat straw (CF/OM ) was carried out to study responses of the soil in microbial abundance and enzyme activity in the Slate Field Research Station of Desert Ecosystem in Fukang, Xinjiang, China. Realtime quantitative polymerase chain reaction (Real-time PCR) analysis indicates that the abundances of soil ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were significantly higher in Treatment CF and Treatment CF/OM than in the control (no application of fertilizer, CK). The population of soil AOB was increased at least by 16 times while that of soil AOA at best by 3 folds, which suggests that AOB play a more important role in in-situ nitrification. Although Treatment CF/OM did not differ much from Treatment CF in crop yield, but was the highest in population size of main soil microbial groups, content of soil organic carbon and activity of most soil enzymes when still sustaining crop yield, which indicates that combined application of chemical fertilizer and organic manure is conducive to maintenance of soil micro-biodiversity and plays an important role in improving soil quality for sustainable agriculture.关键词
长期施肥/灰漠土/微生物丰度/酶活性/荧光实时定量PCRKey words
Long-term fertilization/ Gray desert soil/ Microbial abundance/ Enzyme activity/ Real-time PCR分类
生物科学引用本文复制引用
李晨华,贾仲君,唐立松,吴宇澄,李彦..不同施肥模式对绿洲农田土壤微生物群落丰度与酶活性的影响[J].土壤学报,2012,49(3):567-574,8.基金项目
国家自然科学基金面上项目(40971153)与中国科学院"西部之光"人才培养计划"西部博士资助项目"(XBBS200909)共同资助 （40971153）
