物理化学学报2012，Vol.28Issue(5)：1054-1062,9.DOI:10.3866/PKU.WHXB201202222
烟酸修饰尾式卟啉的合成及其与人血清白蛋白的相互作用
Synthesis of Tailed Porphyrin Modified with Nicotinic Acid and Interactions with Human Serum Albumin
摘要
Abstract
Free porphyrins, namely o-(niacin)C2O-T(3p-OCH3)PP and p-(niacin)C2O-T(3p-OCH3)PP, their complexes, o-(niacin)C2O-T(3p-OCH3)PPZn and p-(niacin)C2O-T(3p-OCH3)PPZn modified with nicotinic acid, were designed, synthesized, and characterized by elementary analysis, and UV-Vis, 1H nuclear magnetic responance (1H NMR), and infrared (IR) spectroscopies. The conformations of the Zn porphyrins were calculated using a quantum- chemical method. The experimental results showed the following. It was found that the nicotinic acid group was on the porphyrin plane in o-(niacin)C2O-T(3p-OCH3)PPZn and Zn -N intramolecular coordination interactions which existed between the N atom of the nicotinic acid group in the side-chain and the Zn2+ in the porphyrin plane. The nicotinic acid group was far from the porphyrin plane in p-(niacin)C2O-T(3p-OCH3)PPZn and Zn - N intermolecular coordination interactions which existed between the N atom of the nicotinic acid group in one Zn porphyrin and the Zn2+ in the other porphyrin plane. The fluorescence properties of the interactions between Zn porphyrins and human serum albumin (HSA) were studied using fluorescence spectroscopy. There is a large quenching interaction between the Zn porphyrins and human serum albumin. The mechanism of the combination reaction is hydrogen bonding or van der Waals interactions between the Zn porphyrins and human serum albumin. The fluorescence quenching data were analyzed using the Stem-Volmer equation and a double-reciprocal equation, and the quenching constant, binding constant, and thermodynamic parameters were obtained.关键词
烟酸/锌卟啉/人血清白蛋白/荧光猝灭/热力学参数Key words
Nicotinic acid/ Zn porphyrin/ Human serum albumin/ Fluorescence quenching/ Thermodynamic parameter分类
化学化工引用本文复制引用
彭玉苓,王树军,傅丽,张成根,刘新刚..烟酸修饰尾式卟啉的合成及其与人血清白蛋白的相互作用[J].物理化学学报,2012,28(5):1054-1062,9.基金项目
河北省自然科学基金(B2010001803) （B2010001803）
廊坊师范学院科学研究项目重点专项基金(LSZZ201003)资助 （LSZZ201003）
