西安交通大学学报（医学版）2012，Vol.33Issue(3)：266-270,5.
纳米氧化锌对大鼠肝及肝癌细胞的毒性效应
Cytotoxicity of ZnO nanoparticles in rat liver cells and hepatocarcinoma cells
摘要
Abstract
Objective To explore the potential cytotoxicity mechanisms of ZnO nanoparticles (NPs) in BRL-3A (rat liver cell line) and CBRH-7919 (rat hepatocarcinoma cell line) cells. Methods We checked cell viability after ZnO exposure at varying concentrations (0. 1 — 100 mg/L) and different exposure periods (12 — 48 h) and changes in reactive oxygen species (ROS) and glutathione (GSH) levels. Results Compared to the NP-free controls, ZnO NPs induced cytotoxicity in concentration-dependent and time-dependent manners in both cell lines. We found high cell viability in CBRH-7919 cells, indicating the better tolerance of CBRH-7919 cells to ZnO NPs than to BRL-3A cells. The increased ROS levels had a negative correlation with reduced cell viability and GSH levels, indicating that ZnO NPs could lead to cytotoxicity through oxidativc stress in normal and cancer cells. Conclusion These results suggest that there exist different degrees of cytotooxicity induced by ZnO NPs in normal and cancer cells, which may deserve consideration in future assessment of nanotoxicity and treatment of cancer.关键词
氧化锌纳米颗粒/细胞毒性/活性氧/谷胱甘肽Key words
ZnO nanoparticle/ cytotoxicity/ reactive oxygen species (ROS)/ glutathione (GSH)分类
生物科学引用本文复制引用
沙保勇,徐峰,卢天健..纳米氧化锌对大鼠肝及肝癌细胞的毒性效应[J].西安交通大学学报（医学版）,2012,33(3):266-270,5.基金项目
国家自然科学基金资助项目(No.10825210,No.11120101002),高校学科创新引智计划项目(No.B06024) （No.10825210,No.11120101002）
