西安交通大学学报（医学版）2012，Vol.33Issue(3)：271-275,5.
T-2毒素对软骨细胞整合素表达的影响及硒的拮抗作用
Effect of T-2 toxin on integrins expression and antagonistic role of selenium
摘要
Abstract
Objective To study the effects of T-2 toxin and selenium on integrins expression to explore the relationship of T-2 toxin and selenium with the etiology of Kashin-Bcck disease. Methods We used MTT assay to assess the proliferation of chondrocytes C-28/I2 treated with different concentrations of T-2 toxin and select the optimal time and concentration of T-2 toxin. Meanwhile, Real-time PCR was used to analyze the expression of integrins treated wih T-2 toxin or selenium. Results T-2 toxin could inhibit the proliferation of chondrocytes C-28/I2. Integrins profile changed with the addition of T-2 toxin; the significantly upregulated integrins were av and Pi, while the downregulated integrins were a2, a5 and P5. Moreover, selenium could partly block the integrins changes. Conclusion T-2 toxin could obviously inhibit the growth of chondrocytes while selenium could protect chondrocytes by antagonizing the expression of integrins.关键词
大骨节病/T-2毒素/软骨细胞/整合素/硒Key words
Kashin-Beck disease/ T-2 toxin/ chondrocyte/ integrin/ selenium分类
医药卫生引用本文复制引用
王加丽,罗明秀,李锦,陈静红,付强,王伟,张增铁,曹峻岭..T-2毒素对软骨细胞整合素表达的影响及硒的拮抗作用[J].西安交通大学学报（医学版）,2012,33(3):271-275,5.基金项目
国家自然科学基金资助项目(No.30872187,31070725) （No.30872187,31070725）
