西安交通大学学报（医学版）2012，Vol.33Issue(3)：287-290,303,5.
氯胺酮对大鼠脊神经结扎神经病理性痛的作用
Effects of ketamine on spinal nerve ligation-induced neuropathic pain
摘要
Abstract
Objective To investigate whether ketamine can prevent microglia activation and thus exert its analgesic effect in neuropathic pain conditions. Methods Based on the spinal nerve ligation (SNL) neuropathic pain model, we first investigated the anti-nociceptive effects of intrathecal (i.t.) ketamine on basal pain threshold and SNL-induced neuropathic pain by behavioral study with von Frey filaments. Then we detected the related microglia activation levels after ketamine administration on SNL or normal rats by immunohistochemistry. Results Behavioral study indicated that ketamine i.t. attenuated SNL-induced mechanical allodynia without affecting the basal paw withdrawal threshold. Immunohistochemistry indicated that ketamine i.t. relieved SNL-induced marked microglia activation in the ipsilateral spinal dorsal horn. Together with the semi-quantification of the immunoreactive density, it was shown that ketamine i.t. inhibited SNL-induced microglia activation by attenuating the up-regulation of OX-42. Conclusion Suppressing nerve injury-induced microglia activation could be a novel mechanism of the anti-allodynia effect of ketamine on neuropathic pain.关键词
氯胺酮/小胶质细胞/神经病理性痛/脊髓/大鼠/脊神经结扎Key words
ketamine/ microglia/ neuropathic pain/ spinal cord/ rat/ spinal nerve ligation (SNL)分类
医药卫生引用本文复制引用
刘蕊,胡文艳,栗茜,每晓鹏,徐礼鲜,吴利平..氯胺酮对大鼠脊神经结扎神经病理性痛的作用[J].西安交通大学学报（医学版）,2012,33(3):287-290,303,5.基金项目
国家自然科学基金资助项目(No.30901400,81100816) （No.30901400,81100816）
