西安交通大学学报（医学版）2012，Vol.33Issue(3)：291-294,4.
运用单克隆抗体分析流感病毒H1亚型HA蛋白的抗原表位
Analysis of epitopes on HA of H1 subtype influenza virus using monoclonal antibodies
摘要
Abstract
Objective To analyze the hcmagglutinin (HA) antigenic epitopes of HI influenza virus. Methods First, we used 97 monoclonal antibodies to HI influenza virus HA antigen and classified antigenic category by means of indirect ELISA. Then, an epitope classification was made through blocking ELJSA test. Finally, the antibodies and antigen binding domain was analyzed by computer modeling to predict the possible binding sites. Results HA epitopes of influenza virus H1 subtype were classified into eight categories according to antibody cross-reactivity. Then HA antigenic epitopes of H1 subtype were divided into fifteen categories according to the preliminary results of ELISA blocking experiments. The results of computer simulation were consistent with the data of blocking ELISA test. Conclusion The paper classified HA antigen of H1N1 subtype of influenza virus into at least fifteen epitopes, and the results of ELISA blocking experiments and computer simulations confirmed with each other. This study provides available experimental data for improving prediction methods of HA epitope of Hl-type influenza virus and the development of universal vaccine.关键词
流感病毒/H1亚型/HA抗原/抗原表位/单克隆抗体Key words
influenza virus/ HI subtype: HA antigen/ antigenic epitope/ monoclonal antibody分类
医药卫生引用本文复制引用
李慧瑾,郭春艳,赵彭花,李研,高锦伟,胡巧侠,李元,胡军..运用单克隆抗体分析流感病毒H1亚型HA蛋白的抗原表位[J].西安交通大学学报（医学版）,2012,33(3):291-294,4.基金项目
"传染病防治"科技重大专项传染病监测技术平台项目(No.2009ZX10004-202) （No.2009ZX10004-202）
