西安交通大学学报（医学版）2012，Vol.33Issue(3)：336-339,364,5.
ICI118551联合吉西他滨对胰腺癌细胞BxPC-3增殖和凋亡的影响及其机制
Effect and mechanisms of ICI 118551 combined with gemcitabine on the proliferation and apoptosis of BxP C-3 cells
摘要
Abstract
To investigate the anti-proliferative effects of P,-adrenoceptor blocker ICI 118551, gemcitabine and ICI 118551 + gemcitabine on BxPC-3 cells and further analyze the underlying mechanisms. Methods MTT was used to detect the anti-prolifcrativc effect of ICI 118551, gemcitabine and ICI 118551 + gemcitabine on BxPC-3 cells. The apoptosis index was determined using TUNEL, and Annexin V and fluorescein isothiocyanate/propidium iodide flow cytometry assay, respectively. Expressions of Bcl-2 and Bax mRNA were detected by RT-PCR. Protein levels of Bcl-2, Bax and NF-kB subunit P65 were analyzed by Western blotting. Results ICI 118551, gemcitabine and ICI 118551 + gemcitabine could suppress proliferation of BxPC-3 cells. ICI 118551 + gemcitabine showed a higher apoptosis rate induction than ICI 118551 or gemcitabine alone. When cells were treated with gemcitabine in combination with ICI 118551, NF-kB activation was blocked; the expression of Bax/Bcl-2 protein was substantially increased. Conclusion ICI 118551 combined with gemcitabine has a synergistic anticancer effect on BxPC-3 cells, and the combination may be an effective therapeutic strategy for pancreatic cancer.关键词
吉西他滨/β2上腺素能受体阻滞剂/胰腺癌/凋亡/ICI 118551/增殖Key words
gemcitabine/ β2-adrenoceptor blocker/ pancreatic cancers apoptosis/ ICI 118551/ proliferation分类
医药卫生引用本文复制引用
单涛,徐军,刘江波,李军涛,李彬,段万星..ICI118551联合吉西他滨对胰腺癌细胞BxPC-3增殖和凋亡的影响及其机制[J].西安交通大学学报（医学版）,2012,33(3):336-339,364,5.基金项目
陕西省13115科技攻关项目资助(No.2010ZDKG-49) （No.2010ZDKG-49）
