西安交通大学学报（医学版）2012，Vol.33Issue(3)：370-373,4.
四味芳香开窍药的挥发性成分对缺血缺氧PC12细胞及细胞内Ca2+的影响
Effects of volatile constituents of four resuscitation-inducing aromatic herbs on oxygen glucOse deprivation in PC12 cells and intracellular Ca2+
摘要
Abstract
To investigate the effects of volatile constituents of four resuscitation-inducing aromatic herbs on sodium dithionote (Na2S2Ot )-induced oxygen glucose deprivation and Ca2+ in differentiated rat pheochromocytofna (PC12) cells. Methods In vitro research, oxygen glucose deprivation model of PC12 cells was established by exposure to Na2S2O4 of 5 mmol/L. The protective effects of volatile constituents of Moschus, Styrax, Benzoinum extraction by water-vapor distilling and Borneolum Syntheticum on the model were evaluated by cytomorphology and colormetric MTT assay. The concentration of intracellular Caz+ was assayed by the determination system. Results Moschus (4 fg/mL, 8 ng/mL and 16 Mg/mL), Borneolun Syntheticum (8 cg/mL and 16^g/mL), Styrax (20 cg/mL and 40 Mg/mL) and Benzoinum (16 fg/mL) markedly increased the optical density at 490 nm tested by colorimetric MTT assay (P<0. 05, P<0. 01) and showed their significant effects in protecting PC12 cells against Na2S2O4-induced apoptosis. Styrax (40 fig/mL and 80 μg/mL) and Benzoinum (4 μg/ mL, 8 μg/mL and l6μg/mL) markedly attenuated the concentration of intracellular Caz+ (P<0.05, P<0.01). Moschus and Borneolun Syntheticum showed the tendency of reducing the concentration of intracellular Ca2+ . Conclusion The volatile constituents of Moschus, Styrax, Benzoinum and Borneolum Syntheticum can markedly protect PC12 cells from oxygen glucose deprivation injury through attenuating the concentration of intracellular Ca'+ and preventing calcium overload.关键词
四味芳香开窍药/挥发性成分/连二亚硫酸钠/PC12细胞/Ca2+Key words
four resuscitation-inducing aromatic herbs volatile constituent/ sodium dithionite/ PC12 cells/ Caz+分类
医药卫生引用本文复制引用
王丹丹,王建,彭颖,王茜,马骁,唐丹霞,夏厚林,曾南..四味芳香开窍药的挥发性成分对缺血缺氧PC12细胞及细胞内Ca2+的影响[J].西安交通大学学报（医学版）,2012,33(3):370-373,4.基金项目
国家重点基础研究发展计划(973计划)(No.2007CB512606) （973计划）
