西北林学院学报2012，Vol.27Issue(3)：109-112,4.DOI:10.3969/j.issn.1001-7461.2012.03.23
雷公藤属内生真菌研究(Ⅱ)昆明山海棠内生真菌的分离鉴定及抗肿瘤活性菌株筛选
Study on the Endophytic Fungi of Tripterygium( Ⅱ ) Isolation of Endophytic Fungi from T.hypoglaucum and Screening of Strains Exhibited Antitumor Activities
摘要
Abstract
Endophytic fungi were isolated from the roots and stems of Tripterygium hypoglaucum by mi-crobial purification methods. The identification of the fungi was carried out by the morphology and microscopic characteristics of the strains. The fermented products of the fungi were extracted by ethyl acetated, and the antitumor activity in vitro of the extracts to 4 tumor cell lines . i. e. , Bel-7402, A549, BGC823, and HCT-8 was examined by MTT assay. Thirty-seven strains of the fungi were isolated from the healthy roots and stems of T. hypoglaucum,belonging to 8 genera, 4 families, and 2 orders. Three of them exhibited significant cytotoxic activities toward the tumor cell lines tested in the MTT assay. It was the first time in China that the endophytic fungi were isolated from T. hypoglaucum, indicating the diversity of the endophytic fungi in T. hypoglaucum, and showing important value of development.关键词
昆明山海棠/内生真菌/抗肿瘤活性/MTT法Key words
Tripterygium hypoglaucum / endophytic fungi/ anti-tumor activity/ MTT assay分类
农业科技引用本文复制引用
王家明,张喜庆,田晓敏,王彩骄,杜万富,孙华,王永禄,李敬华..雷公藤属内生真菌研究(Ⅱ)昆明山海棠内生真菌的分离鉴定及抗肿瘤活性菌株筛选[J].西北林学院学报,2012,27(3):109-112,4.基金项目
天津科技大学实验室开放基金项目(1004A305). （1004A305）
