陕西科技大学学报（自然科学版）2012，Vol.30Issue(2)：25-27,3.
HPLC-ELSD法测定酸羊奶中水苏糖含量
Determination of Stachyose in Goat Yogurt by HPLC-ELSD
宋娟娜 1陈合 1王长凤 1舒国伟1
作者信息
- 1. 陕西科技大学生命科学与工程学院,陕西西安710021
- 折叠
摘要
Abstract
To establish a HPLC-ELSD method for determining the stachyose in goat yogurt. Stachyose was successfully separated on a Hypersil NH2(250 mm ×4. 6 mm,5 μm)column at ambient temperature, the mobile phase composition was Methanol; water (83 : 17). The flow rate was 1. 0 mL/min. The parameters of ELSD were set as follows; Drift tube temperature was 85℃ , Gas pressure was 35 Psi ,Gain was 10. This method fulfilled all the standard requirements of linearity, accuracy and precision. The linear range was 2. 504~12.52 μg (r = 0. 999 8). This method was simple, stable, accurate, sensitive, and reproducible.关键词
酸羊奶/水苏糖/HPLC-ELSD法Key words
goat yogurt/ stachyose/ HPLC-ELSD分类
轻工纺织引用本文复制引用
宋娟娜,陈合,王长凤,舒国伟..HPLC-ELSD法测定酸羊奶中水苏糖含量[J].陕西科技大学学报（自然科学版）,2012,30(2):25-27,3.
