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胰蛋白酶水解鲜猪皮的条件选择

冯成利 党蕊叶 冯慧 刘晓农 权清转

陕西科技大学学报(自然科学版)2012,Vol.30Issue(3):23-25,29,4.
陕西科技大学学报(自然科学版)2012,Vol.30Issue(3):23-25,29,4.

胰蛋白酶水解鲜猪皮的条件选择

Condition selection of the hydrolysis of pigskin with trypsin

冯成利 1党蕊叶 1冯慧 1刘晓农 1权清转1

作者信息

  • 1. 陕西省动物研究所,陕西西安 710032
  • 折叠

摘要

Abstract

In this work, trypsin was used to hydrolyze pigskin in order to avoid the destruction of collagen polypeptide during acid-alkali hydrolysis process. It was found that the hydrolysis degree increased with the increase of the trypsin content. The maximum hydrolysis degree of pigskin was realized at pH 7~8, The initial rate of the hydrolysis reaction was large while it decreased after reacting for 30 minutes. The optimum condition of the hydrolysis of pigskin by trypsin was as follows: pH 7. 5, hydrolysis temperature 48 ℃, ratio of trypsin 40 mg/g, substrates concentration 8%, hydrolysis time 2 h. Under this condition, the hydrolysis degree of pigskin could reach 16%.

关键词

胰蛋白酶/猪皮/胶原多肽/水解

Key words

trypsin I pigskin I collagen polypeptide/ hydrolysis

分类

轻工纺织

引用本文复制引用

冯成利,党蕊叶,冯慧,刘晓农,权清转..胰蛋白酶水解鲜猪皮的条件选择[J].陕西科技大学学报(自然科学版),2012,30(3):23-25,29,4.

基金项目

陕西省科学院科技计划项目(2009K-02) (2009K-02)

陕西省科学院科技计划项目(2006K-19) (2006K-19)

陕西科技大学学报(自然科学版)

OACSTPCD

2096-398X

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