现代妇产科进展2012，Vol.21Issue(4)：246-249,254,5.
带hTERT启动子的腺病毒干扰ERCC1基因表达逆转卵巢癌顺铂耐药的研究
Interfering expression of ERCC1 by adenovirus with hTERT promoter reverses cisplatin resistance in ovarian cancer cell lines
刘微 1毕秋英 1瞿全新1
作者信息
- 1. 天津医科大学一中心临床学院,天津300070
- 折叠
摘要
Abstract
Objective:To study the effect of interfering the expression of ERCC1 by ad-enovirus with hTERT on reversing cisplatin resistance in ovarian carcinoma cell lines. Methods : Recombinant adenovirus were divided into 1MOI, 10MOI, 50MOI, 80MOI to infect SK-OV3 ,SKOV3/DDP and ECV304,while the same dose of empty carrier adenovirus and uninfcct-od cells as control group. The expression of ERCC1 mRNA was detected by RT-PCR and the expression of ERCC1 protein was detected by Western blot in recombinant adenovirus- transfec-tion cells and the control groups. The cisplalin chemotherapeutic sensitivity was detected by MTT assay. Results: (l)The expressions of ERCC1 mRNA and ERCC1 protein in SKOV3 and SKOV3/DDP infected with various doses of Ad-hTERT-ERCCl shRNA were declined compared with uninfected groups (P<0. 01 ). The expressions of ERCC1 mRNA and ERCC1 protein in ECV304 cell and cells transfecled with empty vector did not changed compared with the uninfected groups (P>0.05). (2)Cisplatin sensitivity in SKOV3/DDP was efficiently enhanced after infected with recombinant adenovirus(P<0. 01) ,and was dose-dependent. Conclusion; The adenovirus with hTERT promoter can reverse the resistance of cisplatin by interfering the expression of ERCC1 in SKOV3/DDP.关键词
卵巢肿瘤/顺铂耐药/ERCC1/重组腺病毒Key words
Ovarian neoplasms/Cisplatin resistance/Excision repair cross complementation group 1/ Recombinant adenovirus分类
医药卫生引用本文复制引用
刘微,毕秋英,瞿全新..带hTERT启动子的腺病毒干扰ERCC1基因表达逆转卵巢癌顺铂耐药的研究[J].现代妇产科进展,2012,21(4):246-249,254,5.
