现代妇产科进展2012，Vol.21Issue(4)：280-285,6.
改良筛网法分离培养人子宫内膜细胞
A modified filter method to isolate and culture human endometrial cell
摘要
Abstract
Objective;To establish a modified method to isolate endometrial glandular epithelial cells and stromal cells with higher purity and greater number in vitro. Methods: The fresh endometrium of 1 mg from 12 endometrial specimens were obtained,digested with 0. 25% collagenase I containing 0.005% DNA enzyme and then seperated by filter method and modified method which refers to combination serial filtration with gradient centrifugation, respectively. The cell number was record after separation by cell count. The purity of the isolated endometrial glandular epithelial cells and stromal cells were identified morphologically by light microscopy, and stained for vimentin and cytokeratins by immunohistochemistry and immumofluores-cent assay. Results: The cells were isolated and cultured from 11 endometrial specimens successfully with both methods. (8.09 ± 1.24)×105 epithelial cells and (27.23 ± 0.50)× 105 stromal cells with the purities of (91.95 ± 0.47)% and (99.28 ± 0.21)% respectively were isolated from endometrial tissues using the modified method,whereas (0.47 ± 0.09)×105 epithelial cells and (23.73 ± 5.37)×105 stromal cells with the purities of (89.54 ± 3.75)% and (92. 82 ± 1.60)% respectively were obtained by filter method. Compared to previous filter methods, the purity of glandular epithelial cells and cell count of stromal cells by modified method were significantly higher than those by filter method,respectively (P= 0. 000). Conclusion; We have developed a modified method to isolate endometrial glandular epithelial and stromal cell populations with a higher purity and greater number in vitro.关键词
子宫内膜/腺上皮细胞/间质细胞/细胞培养Key words
Endometrium/Glandular epithelial cell/Stromal cell/Cell culture分类
医药卫生引用本文复制引用
陈淑琴,金文艳,黄佳明,姜红叶,姚书忠..改良筛网法分离培养人子宫内膜细胞[J].现代妇产科进展,2012,21(4):280-285,6.基金项目
国家自然科学基金面上项目(No:81070472) （No:81070472）
