现代妇产科进展2012，Vol.21Issue(7)：516-519,4.
慢病毒介导的EZH2靶向RNA干扰对人卵巢癌SKOV3细胞增殖的影响
The impact of lentivirus-based EZH2 RNAi on the proliferation of human ovarian carcinoma SKOV3 cells
摘要
Abstract
Objective: To explore the proliferation of human ovarian carcinoma SKOV3 cells under inhibition of zeste homolog 2 by lentivirus-based EZH2 RNAi. Methods: We designed lentivirus-based RNA interference ( RNAi) , synthesized and transfected SKOV3 cells to inhibit EZH2 expression. Cell proliferations and growth were evaluated with Cell Counting Kit 8 ( cck-8). Expressions of EZH2 ,PCNA mRNA and protein level were determined using RT-PCR and Western bolt respectively. Results; Comparing with blank group and empty transfection group, EZH2-shRNA transfection could suppress SK0V3 cells proliferation and growth (P < 0.05), and downregulate EZH2,PCNA mRNA expression and protein levels ( P<0. 05, P< 0.05) further. Conclusion: The lentivirus-EZH2 RNAi can restrain proliferation of SKOV3 cells,so silencing EZH2 may be a new way of gene therapy.关键词
卵巢肿瘤/RNA,小分子干扰/慢病毒属/细胞增殖/EZH2/SKOV3Key words
Ovarian neoplasms/ RNA, small interfering/ Lentivirus/ Cell proliferation/EZH2/SKOV3分类
医药卫生引用本文复制引用
闫忠鑫,翟小龙,韩凤娟,丁涛,蔡冬燕,赵立军..慢病毒介导的EZH2靶向RNA干扰对人卵巢癌SKOV3细胞增殖的影响[J].现代妇产科进展,2012,21(7):516-519,4.基金项目
黑龙江省博士后资助项目(No:LBH-Z09008) （No:LBH-Z09008）
黑龙江省青年科学基金资助(No:QC08C37) （No:QC08C37）
