现代妇产科进展2012，Vol.21Issue(7)：525-529,5.
卵巢癌相关成纤维细胞与卵巢癌淋巴结转移的关系
The relationship between ovarian cancer associated fibroblasts and lymph node metastases in ovarian cancer
黄在菊 1唐慧娟 1王泽华1
作者信息
- 1. 华中科技大学同济医学院附属协和医院妇产科,武汉430022
- 折叠
摘要
Abstract
Objective: To investigate the relationship between the amount of CAFs (cancer-associated fibroblasts) and the lymph node metastases in ovarian cancer and evaluate the role of CAFs in lymphangiogenesis and lymphatic endothelial cells ( LEG) proliferation and migration. Methods:Using immunohistochemical method and computer-assisted image analysis, we quantified CAFs in 71 human ovarian cancer using a-SMA as a marker. Lymph vessel density (LVD) was measured by quantitative immunohistochemistry using anti-D2-40 antibody, EdU incorporation assay and Transwell migration assay were conducted to evaluate the proliferation and motility of EOC cell lines cocultured with CAFs. Results;The CAFs in lymph-node positive malignancies was more than that in lymph-node negative malignancies, (29. 39±4, 32)% and (22. 56±6. 78)% respectively(P = 0.024). The lymphatic vessel density was positively correlated with the number of CAFs and has statistic significance. By co-cultivation with CAFs, the number of dividing or magration LEC increased 2. 8-fold (P<0. 0001) and 5. 2-fold (P< 0.0001) respectively. Conclusion: CAFs in ovarian cancer seem to play an important role in the ovarian cancer development and lymph node metastases through induced lymphangiogenesis and LEC proliferation and migration.关键词
卵巢肿瘤/成纤维细胞/淋巴转移/淋巴管生成/淋巴管内皮细胞Key words
Ovarian neoplasms/ Fibroblasts/ Lymphatic metastasis/ Lymphangiogenesis / Lymphatic endothelial cells分类
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黄在菊,唐慧娟,王泽华..卵巢癌相关成纤维细胞与卵巢癌淋巴结转移的关系[J].现代妇产科进展,2012,21(7):525-529,5.
