现代妇产科进展2012，Vol.21Issue(7)：530-535,6.
CXCR7及其配体在子宫内膜癌中的表达与意义
Expression and significance of CXCR7 chemokine receptor and its ligands in endometrial carcinoma
摘要
Abstract
Objective:To exame the expression of CXCR7 chemokine receptor and its ligands in endometrial carcinoma tissues and cell lines, and explore whether estrogen can regulate the expression of CXCR7, Methods: RT-PCR was firstly used to analyze the transcription levels of twenty chemokine receptors in Ishikawa(ERa positive) and AN3CA(ERa negative) cell lines, and the chemokine receptor which was expressed highly was selected . The expression of this chemokine receptor and its ligands in endometrial carcinoma tissues and cell lines were examed by immunocytochemical staining, and the effect of estrogen on the chemokine receptor protein levels was explored by Western blot. Results: (1) GXCR7 mRNA was highly expressed in Ishikawa and AN3CA cell lines. Immunocytochemical staining suggested that CXCR7 and its ligand SDF-1 were expressed in endometrial carcinoma tissues and Ishikawa, AN3 CA cell lines, while no expression of the another ligand I-TAC;(2)In Ishikawa,estrogen could up-regulate the protein level of CXCR7 with the changes of concentration,otherwise in AN3CA,the expression of CXCR7 was not notablely changed by the stimulation of estrogen. Conclusions: CXCR7/SDF-1 is highly expressed in endometrial carcinoma tissues and cell lines. Estrogen can up-regulate the expression of CXCR7. CXCR7/SDF-1 seems to act as an important regulator in the endometrial carcinoma.关键词
子宫内膜癌/雌激素/趋化因子受体/CXCR7/SDF-1/I-TACKey words
Endometrial carcinoma/ Estrogens/ Chemokine, receptors/ CXCR7/ SDF-1/ I-TAC分类
医药卫生引用本文复制引用
顾红琴,贺银燕,赵贵旭,赖允丽,王倩倩,孙姗姗,席晓薇,张箴波,丰有吉..CXCR7及其配体在子宫内膜癌中的表达与意义[J].现代妇产科进展,2012,21(7):530-535,6.基金项目
国家自然科学基金青年项目(No:81001154/H1621) （No:81001154/H1621）
上海市科委青年科技启明星计划(No:11QA1405300) （No:11QA1405300）
