现代科学仪器Issue(3)：60-63,4.
常规荧光法与同步荧光法用于浒苔叶绿素a测定的比较研究
Comparison of Two Fluorometric Methods for Chlorophyll-a Measurement in Enteromorpha Prolifera
摘要
Abstract
Determinations of Chlorophyll a in Enteromorpha prolifera were compared using two methods of conventional fluorescence spectrometry and synchronous fluorescence spectrometry, N,N-dimethylformamide （DMF） was used for the extraction of chlorophyll-a （Chl a） in Enteromorpha prolifera. The results showed that DMF was a shorter amount of time, more effective and more convenient than 90% aceton solvent in extracting of Chl a. There was no significant difference between two fluorometric methods for Chl a concentration measurement. The content of measured Chl a ranged from 4.38 to 9.35 mg/g for conventional fluorometfic method and from 4.53 to 8.74 mg/g for synchronous fluorometric method, respectively, for 20 Enteromorpha prolifera samples from four stations, Qingdao.关键词
常规荧光法/同步荧光法/浒苔/叶绿素a/N,N-二甲基甲酰胺Key words
Fluorescence/Synchronous fluorescence/Enteromorpha prolifera/Chlorophyll a/N,N-dimethylformamide分类
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夏滨,郑晓玲,何鹰..常规荧光法与同步荧光法用于浒苔叶绿素a测定的比较研究[J].现代科学仪器,2012,(3):60-63,4.基金项目
国家863计划项目 （）
