新疆农业科学2012，Vol.49Issue(4)：701-707,7.DOI:10.6048/j.issn.1001-4330.2012.04.016
一株棉秆纤维素分解真菌的分离筛选及酶学性质研究
Isolation of a Cotton Stalk Cellulose Decomposing Fungus and Its cellulase Properties
摘要
Abstract
[Objective] The purpose of this project was to study cellulase properties of a cellulose decomposing fungus SJ - 1 isolated from cotton stalk decomposing products were studied. [Method] Effects of temperature, pH value and metal ions on cellulase activity were studied, and Michaelis constant Km of enzymatic reaction and the maximum reaction rate Vmax were examined by Lineweaver - Burk method. [ Result]The best reaction temperature to CMCase and FPase was ranged from 50 to 60℃, and the best pH value was 7. 0, which showed the tolerance to high temperature and alkali. K+ and Fe2+ activated the cellulase activity significantly, while Cu2 + , Mg2 + , Ca2+ and Al3 + showed certain inhibition, the inhibition of Al3+ exhibited much more strongly, Cu2+ inhibited FPase strongly as well, Mn2+ showed activation to FPase but inhibition to CMCase, Zn2 + had no influence on cellulase activity. When CMC - Na was taken as substrate, Km and Vmax of the enzymatic reaction were 2.69 mg/mL and 0.53 mg/( mL·min) respectively. [Conclusion] The strain showed good properties of cellulase activity, and provided references for further breeding and alteration of this fungus.关键词
棉秆/腐解/纤维素分解菌/纤维素酶/酶学性质Key words
cotton stalk/decomposition/cellulose decomposing microorganism/cellulase/enzymatic properties分类
农业科技引用本文复制引用
白宝伟,张琴,滕立平,杨瑛,李艳宾..一株棉秆纤维素分解真菌的分离筛选及酶学性质研究[J].新疆农业科学,2012,49(4):701-707,7.基金项目
国家自然科学基金(30960071) （30960071）
