新疆农业科学2012，Vol.49Issue(6)：1120-1126,7.DOI:10.6048/j.issn.1001-4330.2012.06.021
紫外线诱变选育高产醋酸的醋酸菌研究
Study on Breeding of Mutants of High -yield Acetic Acid bacteria by Ultraviolet Radiation
摘要
Abstract
[Objective]The purpose of this study was to breed acetic acid bacteria with high - yield acetic acid for brewing vinegar from apricot dregs. [Method] The growth curve of acetic acid bacteria C1-0 was determined, then the strain of C1-0 was mutated by ultraviolet irradiation . And the lethal rate of mutants was set 85% -90% , The high -yield strains of acetic acid bacteria were screened. By means of the experiments of determining both pH value and acid content, the acid production for 5 generations of mutants was measured. [Result] The strain of C1-0 was irradiated for 20s by UV when it came into logarithmic growth phase after it was incubated for 24 h, and, the lethal rate was 88.72%. Three high - yield mutants of C1-11, C1-27 and C1-30 have been obtained. The acid yield of C1-11 , C1-27 and C1-30 were 37.29, 38. 80 and 36.60 g/L respectively. The acid yield for 5 generations of mutants changed little . [ Conclution ] The mutants of C1-11,C1-27,C1-30 with high -yield acetic acid by ultraviolet radiation were obtained, the acid yield of C1-11, C1-27,C1-30 were increased by 4.5% , 8.6% , 2.6% than C1-0,and genetic stability was good.关键词
紫外线诱变/选育/醋酸菌/突变株Key words
ultraviolet radiation/ breeding/ acetic acid bacteria/ mutant分类
轻工纺织引用本文复制引用
张蕾,郭龙,傅力..紫外线诱变选育高产醋酸的醋酸菌研究[J].新疆农业科学,2012,49(6):1120-1126,7.基金项目
新疆维吾尔自治区科技计划项目(200931101-5) （200931101-5）
国家"十二五"科技支撑计划项目(2011BAD27B02-04) （2011BAD27B02-04）
