厦门大学学报（自然科学版）2012，Vol.51Issue(3)：391-396,6.
靶向肿瘤多药耐药基因mdr 1的siRNA的构建与鉴定
Construction and Identification of siRNA Targeting to Multidrug Resistance Gene 1
摘要
Abstract
The over-expression of mdr 1 gene is the main reason of multidrug resistance(MDR) of tumor cells. Inhibiting the expression of mdr 1 gene can be used to reverse the MDR. RNA interfenceCRNAi) is a sequence-specific intracellular mechanism of gene silence. In this study,the construction of small interfering RNAs(siRNAs) element against mdrl gene effectively and specifically were explored. Three siRNA sequences targeting to mRNA stem or loop stucture separately were selected based on the sequence of mdr 1 gene. Using various reporter plasmids containing different target sequences, the inhibition efficiencies and specificities of the constructs against mdr 1 gene were determined and siMDRIB targeting to the loop structure was selected from the candidates. siMDRIB expression vector was then co-transfected with the mdr 1 gene expression vector in cells,and the expression level of P-gp protein encoded by mdr 1 gene was analysed by immune flow cytometry. Results showed that P-gp protein expression can be effectively inhibited by siMDRIB in transformed cells comparing to control cells. CCK-8 assay was used for cell viability analysis in this study,and results showed that cell viability was not affected by siMDRIB expression in transformed cells. In conclusion,siMDRIB is a promising candidate for the next research on MDR reverse.关键词
mdr 1基因/RNA干扰/siRNAKey words
mdr 1 gene/RNA interference/siRNA分类
医药卫生引用本文复制引用
林燕真,张雅丽,李瑞银,杨连威,程通,温兰玲..靶向肿瘤多药耐药基因mdr 1的siRNA的构建与鉴定[J].厦门大学学报（自然科学版）,2012,51(3):391-396,6.基金项目
福建省卫生厅青年科研课题计划项目(2008-1-53) （2008-1-53）
厦门市卫生局基金项目(3502z20089012) （3502z20089012）
