厦门大学学报（自然科学版）2012，Vol.51Issue(4)：734-739,6.
反相高效液相色谱法分析盘基网柄菌对氨基酸的利用
Analysis of Amino Acid Consumption During the Cultivation of Dictyostelium discoideum by Reversed-phase High Performance Liquid Chromatography
摘要
Abstract
In order to observe amino acid consumption during cultivation of Dictyoslelium discoideum on synthetic SIH medium, a method for determination of amino acids by reversed-phase high performance liquid chromatography(RP-HPLC) with 2, 4-dinitro-chlorobenzene derivatization was investigated. 16 amino acids could be well separated in 60 min,and a good linear relationship between peak area and concentration of amino acids was established with the correlation coefficients in the range of 0. 992-0. 999, Analysis results of samples of D. Discoideum on SIH medium indicated that lysine was completely consumed after 7 d of cultivation. Methionine, tryptophan.arginine and histidine were also utilized considerably while aspartic acid, glutamic acid, glycine, threonine, proline and valine did not show significant demand during the cell growth. This metabolic characteristic would supply reliable data to design a more reasonable synthetic medium for D. Discoideum.关键词
盘基网柄菌/反相高效液相色谱法/氨基酸/2,4-二硝基氯苯Key words
Dictyostelium discoideum/ reversed-phase high performance liquid chromatography> amino acids 2, 4-dinitrochloro-benzene分类
生物科学引用本文复制引用
黄霄红,王颖,叶美玲,罗臻,宋娟,凌雪萍..反相高效液相色谱法分析盘基网柄菌对氨基酸的利用[J].厦门大学学报（自然科学版）,2012,51(4):734-739,6.基金项目
国家自然科学基金项目(31070296) （31070296）
