畜牧兽医学报2012，Vol.43Issue(7)：1150-1155,6.
甲砜霉素在鸡肌肉中的残留消除规律
Thiamphenicol Residue Depletion in Chicken Muscle
摘要
Abstract
A study on thiamphenicol (TAP) residue depletion was conducted in chicken muscle. TAP was extracted from chicken muscle with acetone and dichloromethane. The extract solution was degreased in n-hexane saturated with acetonitrile, dried in nitrogen evaporator and residues were dissolved in mobile phase. TAP was determinated by high performance liquid chromatography (HPLC) with fluorescence detector. The limits of detection (LODs) was 1. 5 μg · kg-1 (S/N = 3) and the limits of quantitation(LOQs)was 5 μg · kg-1(S/N = 10) for TAP in chicken muscle, respectively. After the chickens orally administered successively TAP capsules of 10. 0, 20. 0 and 50. 0 mg · kg-1 of body weight once every day for 5 days, respectively, residues of TAP in chicken muscle had a rise trend. The maximum residue of TAP was detected at the first day after withdrawal time in chicken muscle. At the early days after withdrawal time, the residues of TAP in chicken muscle were eliminated faster, but they were eliminated slowly at the later period. Residues of TAP in chicken muscle were all lower than 50 μg · kg-1 at the 5th withdrawal day, and all lower than the lowest LODs (1. 5 μg · kg-1)at the 9 th withdrawal day. Residues of TAP in chicken muscle were positively correlated with TAP orally administered doses.关键词
鸡肌肉/甲砜霉素/残留/消除/高效液相色谱荧光检测法Key words
chicken muscle/ thiamphenicol/ residue/ depletion/ HPLC with fluorescence detection分类
农业科技引用本文复制引用
谢恺舟,张小杰,陈学森,谢星,孙瑛瑛,张跟喜,王金玉,陈国宏..甲砜霉素在鸡肌肉中的残留消除规律[J].畜牧兽医学报,2012,43(7):1150-1155,6.基金项目
江苏省科技成果转化专项(BA2008071) （BA2008071）
国家肉鸡产业技术体系专项(CARS-42-G23) （CARS-42-G23）
江苏高校优势学科建设工程资助项目 （）
