畜牧兽医学报2012，Vol.43Issue(7)：1170-1176,7.
microRNA-155在长白猪和通城猪骨骼肌发育过程中的表达分析
Expression Analysis of microRNA-155 in Skeletal Muscle at Different Development Stages in Landrace and Tongcheng Pigs
摘要
Abstract
The aim of this study was to detect the expression of miR-155 in skeletal muscle development in Landrace (lean-type) and Tongcheng (obese-type) pigs, and explore the roles of miR-155 in muscle development. Based on the skeletal muscle tissues of 22 stages (12 prenatal and 10 postnatal stages) in Landrace and Tongcheng pigs, respectively, stem-loop RT-PCR was performed to detect the miR-155 expression. Furthermore, the expression of miR-155 in various tissues of adult Tongcheng pigs was detected. The miR-155 differential expression in skeletal muscle development exhibited different expression patterns between Landrace and Tongcheng pigs. In adult Tongcheng pigs, miR-155 was abundantly expressed in small intestines and lung, moderately in heart and placenta, and weakly in other collected tissues. The result indicate that miR-155 may be involved in myofibre proliferation and differentiation and further contribute to skeletal muscle development and muscle phenotype with different genotypes.关键词
猪/miR-155/骨骼肌/发育Key words
pig/ miR-155/ skeletal muscle/ development分类
农业科技引用本文复制引用
赵拴平,昝林森,唐中林,李奎..microRNA-155在长白猪和通城猪骨骼肌发育过程中的表达分析[J].畜牧兽医学报,2012,43(7):1170-1176,7.基金项目
国家自然科学基金(30830080 （）
31171192) （）
国家重大科学研究计划(2009CB941604) （2009CB941604）
