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一株传染性法氏囊病病毒强毒株的分离与鉴定

刘维婷 廖梦 曹瑞兵

畜牧与兽医2012,Vol.44Issue(5):10-13,4.
畜牧与兽医2012,Vol.44Issue(5):10-13,4.

一株传染性法氏囊病病毒强毒株的分离与鉴定

Isolation and identification of a virulent strain of infectious bursal disease virus

刘维婷 1廖梦 1曹瑞兵1

作者信息

  • 1. 南京农业大学动物医学院,江苏南京210095
  • 折叠

摘要

Abstract

Infectious bursal disease (IBD) , caused by infectious bursal disease virus (IBDV) , is an important infectious disease. In the present study, the samples from a suspected chickcn farm in Jiangsu province were detected by RT-PCR using IBDV vp4 specific primers, combined with the clinical symptoms, pathological changes, SPF embryo inoculation test and agar diffusion test. IBDV infection was confirmed in this farm. According to the results of artificial challenge test and the VP4 gene sequence analysis, the IBDV isolate is a virulent strain with VP4 gene variation. The result provides a useful reference for the prevention and control of IBD in Jiangsu province.

关键词

传染性法氏囊病病毒/分离/鉴定/毒力

Key words

infectious bursal disease virus/ isolation/ identification/ virulence

分类

农业科技

引用本文复制引用

刘维婷,廖梦,曹瑞兵..一株传染性法氏囊病病毒强毒株的分离与鉴定[J].畜牧与兽医,2012,44(5):10-13,4.

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