摘要
Abstract
To produce monoclonal antibodies ( McAb) against VP2 protein of porcine parvovirus ( PPV) and to establish an antigen capture ELISA ( AC-ELISA) , BALB/c mice were immunized with VP2 recombinant protein and the mouse splenic cells were fused with SP2/0 cells. The hybridoma cell designated 3C4 and 5F8 was screened by ELISA coated with the PPV antigen. AC-ELISA was developed for detection of PPV using polyclonal antibody as capture antibody and McAb 5F8 as detecting antibody. The AC-ELISA showed no cross-reaction with other five swine viruses, including Japanese encephalitis virus (JEV) , porcine epidemic diarrhea virus (PEDV) , pseudorabies virus (PRV) , porcine reproductive and respiratory syndrome virus (PRRSV) , and classical swine fever virus (CSFV). Comparing with RT-PCR, the concordance was 93. 6% , the sensitivity was 90. 9% , and the specificity was 94. 4%. Therefore, The PPV AC-ELISA has good specificity, sensitivity and repeatability, and could be used for diagnosis of PPV infections.关键词
猪细小病毒/VP2蛋白/单克隆抗体/抗原捕捉ELISAKey words
porcine parvovirus/ VP2 protein/ monoclonal antibodies/ antigen capture ELISA
分类
农业科技
