畜牧与兽医2012，Vol.44Issue(7)：18-22,5.
动物产品及饲料中牛源和羊源性成分三重荧光PCR检测方法的建立
Establishment of multiplex fluorescent PCR assay for the detection of bovine,goat and sheep- derived materials in animal products and feeding stuffs
赵冉 1蔡振鸿 2陈永锋1
作者信息
- 1. 厦门市农产品质量安全检验测试中心,福建厦门361012
- 2. 新奥(厦门)农牧发展有限公司,福建厦门361000
- 折叠
摘要
Abstract
In this study, a multiplex fluorescent PCR assay capable of detecting and differentiating bovine, goat and sheep-derived materials was developed using specific primers and TaqMan probes designed according to mitochondrial DNA gene sequences. The multiplex fluorescent PCR reaction system was optimized. The developed method could successfully detect bovine, goat and sheep-derived materials of 16 animals. The result showed that the real-time PCR can work accurately and efficiently, and can be 100 times as sensitive as conventional PCR of GB/T 20190-2006. Therefore, this method has the potential application for detection of bovine, goat and sheep-derived materials in feed, meat products, milk products, etc.关键词
线粒体DNA/荧光PCR/牛、山羊和绵羊源性成分Key words
mitochondrial DNA/ multiplex fluorescent PCR/ bovine and goat and sheep-derived materials分类
农业科技引用本文复制引用
赵冉,蔡振鸿,陈永锋..动物产品及饲料中牛源和羊源性成分三重荧光PCR检测方法的建立[J].畜牧与兽医,2012,44(7):18-22,5.
