新医学2012，Vol.43Issue(6)：376-379,4.DOI:10.3969/g.issn.0253-9802.2012.06.008
川芎嗪抑制人视网膜母细胞瘤SO-RB50生长机制的研究
Effects of tetramethylpyrazine on human retinoblastoma cell line SO-RB50 and its mechanisms
摘要
Abstract
Objective: To investigate the effect of tetrame-thylpyrazine (TMP) on cell proliferation, CXCR4 expression, cell cycle phase and apoptosis of human retinoblastoma cell line SO-RB50 and to elucidate its possible molecular mechanisms. Methods: SO-RB50 cells were treated with TMP for 48 hours or 72 hours. Methylthiazolyl tetrazolium assay was performed lo test the inhibitory effect of TMP on cell proliferation. Quantitative Real-time (RT-PCR) and western blot were adopted to evaluate CXCR4 expression on SO-RB50 cells. Flow cytometry was used to detect the apoptosis and cell cycle of the SO-RB50 cell line. Results: MTT showed that TMP remarkably inhibited cell viability and growth of SO-RB50 cells ( P < 0.01). RT-PCR and Western blot results showed that TMP down-regulated CXCR4 expression in SO-RB50 cells both at RNA and protein levels. Flow cytometry demonstrated that TMP increased the rate of G0/G1 of SO-RB50 cell cycle (P <0. 01). Conclusions: TMP can inhibit the viability and growth of human retinoblastoma SO-RB50 cell line. To our knowledge, this is the first study to reveal the mechanism of TMP treatment involving the inhibition of chemokine and CXCR4 expression in SO-RB50.关键词
川芎嗪/人视网膜母细胞瘤/细胞周期/CXCR4Key words
Tetramethylpyrazine/ SO-RB50/ Cell cycle/ CXCR4引用本文复制引用
陈朝,潘雪珂,余克明,Joseph M. Kaminski,庄菁..川芎嗪抑制人视网膜母细胞瘤SO-RB50生长机制的研究[J].新医学,2012,43(6):376-379,4.基金项目
国家自然科学基金(30872811) （30872811）
