医药导报2012，Vol.31Issue(7)：831-834,4.DOI:10.3870/yydb.2012.07.001
异鼠李素抑制耐吉非替尼人肺癌细胞PC9增殖的研究
Isorhamnetin Suppresses the Growth of Gefitinib Resistant Human Lung Cancer PC9 Cells
摘要
Abstract
Objective To investigate the role of isorhamnetin on the growth of PC9-IR cells. Methods The gefitinib-resistant PC9(PC9-1R) cells were generated by consistently culturing the PC9 cells with 10% FBS/DMEM contained 0.5 μmol·L-1 gefitinib. Then, the PC9-1R cells were treated with isorhamnetin at different concentrations. The proliferation was detected by MTT assay after 24,48 and 72 h, respectively, with PBMNCs as control. Western blot assay was used to detect the change of growth signal pathway. Additionally, clonal assay was performed to analyze the clone formation ability of PC9-IR with or without isorhamnetin. Results Isorhamnetin inhibitted the growth of PC9-1R. The IC50 at the three time points were 212 μmol·L-1 (24 h) ,98 μmol·L-1(48 h) ,64 μmol·L-1(72 h) ,and PBMNCs was 204 μmol·L-1(72 h). It was showed thai the phosph-Akt was suppressed and the clonal formation ability of PC9-IR was lowered by isorhamnetin. Conclusion Isorhmantin could inhibit the growth of PC9-IR cells through suppressing the phosphorylation of Akt.关键词
异鼠李素/肺癌细胞/吉非替尼/耐药Key words
Isorhamnetin/ Lung cancer cells/ Gefitinib/ Resistance分类
医药卫生引用本文复制引用
李川,杨熹,胡俊波,廖家智..异鼠李素抑制耐吉非替尼人肺癌细胞PC9增殖的研究[J].医药导报,2012,31(7):831-834,4.基金项目
国家973计划(2009CB521802) （2009CB521802）
国家自然科学基金(30872472,30973496,30800569) （30872472,30973496,30800569）
湖北省自然科学基金(2008CDB174,2009CDB239) （2008CDB174,2009CDB239）
中央高校基本科研业务费专项资金(2011JC062,2011JC063) （2011JC062,2011JC063）
华中科技大学自主创新基金(2010MS027) （2010MS027）
