医药导报2012，Vol.31Issue(7)：870-873,4.DOI:10.3870/yydb.2012.07.012
肉桂油抗柯萨奇病毒B3的血清药理学研究
Serum Pharmacological Study of Oleum Cinnamomi on Coxsackievirus B3
摘要
Abstract
Objective To investigate the effects and mechanism of oleum cinnamomi (OC) on primary rat myocardial cells infected with Coxsackie virus B3(CVB3 ) by the serum pharmacological method. Methods The primary rat myocardial cells from the ventricle muscle of new bom Sprague-Dawley rats were prepared. After 72 hours, different doses of OC contained sera obtained via i. v. injection with OC in rats were added in myocardial cells infected with CVB3, to establish the model and control groups at the same lime. The cinnamaldehyde and cinnamic acid in sera were detected at different time points. And the relative content of CVB,mRNA was assayed by Real-time PCR. The survival rate of myocardial cells was determined through a metabolic 3-( 4, 5-dimethylthiazol-2-yl )-2, 5-diphenyl-tetrazolium bromide ( MTT) assay. A simple correlation test was implemented to analyze the relationship by using the SPSS software. Results The results showed that the effect of OC contained serum on the myocardial cell survival rate and relative amount of CVB3 mRNA was contributed to cinnamic acid P< 0.05 or P<0. 01) rather than cinnamaldehyde. Conclusion Oleum cinnamon is an effective antiviral agent against viral myocarditis, the cinnamic acid is the main effective component for which.关键词
肉桂油/桂皮醛/心肌炎,病毒性/血清药理学Key words
Oleum cinnamomi/ Cinnamaldehyde/ Myocarditis, viral/ Serum pharmacology分类
医药卫生引用本文复制引用
丁媛媛,邱麟,曾明,许茜,许景峰..肉桂油抗柯萨奇病毒B3的血清药理学研究[J].医药导报,2012,31(7):870-873,4.基金项目
国家自然科学基金资助项目(20872180) （20872180）
