医药导报2012，Vol.31Issue(8)：990-993,4.DOI:10.3870/yydb.2012.08.007
F1013人血浆蛋白结合率测定及其影响因素
Study on the Determination of Human Plasma Protein Binding Ratio of the Caspase Inhibitor F1013 and Its Influence Factors
吴姗 1魏俊霞 2王琼琼 2吴健鸿 2刘焱文 2曾繁典1
作者信息
- 1. 湖北中医药大学中药资源与中药复方省部共建教育部重点实验室,武汉430061
- 2. 武汉市食品药品检验所,430012
- 折叠
摘要
Abstract
Objective The ultrafiltration was employed to determine the human plasma protein binding ratio of a novel Caspase inhibitor F1013. The influence factors of this determination were investigated. Methods The unbound fractions in plasma were separated with ultrafiltration equipment under different conditions and were determined with high performance liquid chromatography (HPLC). Results The Amicon Ultra-0.5 equipment was suitable for researching the plasma protein binding ratio of F1013. The plasma protein binding ratio of F1013 was increased with the increasing of rotation speed and centrifuge time, and it was decreased with prolongated incubation time. Conclusion Human plasma protein binding ratio of F1013 was influenced by rotation speed, centrifuge time and incubation time. When the ultrafiltration was applied to study the human plasma protein binding ratio of F1013, the best ultrafiltration conditions are that the human plasma spiked with F1013 was incubated in the 37 ℃ water for 1 h, and then put into the Amicon Ultra-0. 5 equipment, to centrifuge at 14 000 r · min~ for 15 min. The method has satisfying recovery, precision, and a good feasibility.关键词
F1013/血浆蛋白结合率/影响因素Key words
F1013/ Plasma protein binding ratio/Influence factors分类
医药卫生引用本文复制引用
吴姗,魏俊霞,王琼琼,吴健鸿,刘焱文,曾繁典..F1013人血浆蛋白结合率测定及其影响因素[J].医药导报,2012,31(8):990-993,4.
