医药导报2012，Vol.31Issue(8)：1057-1058,2.DOI:10.3870/yydb.2012.08.030
高效液相色谱法测定足宁颗粒中盐酸小檗碱含量
Determination of Berberine Hydrochloride in Zuning Granules by HPLC
郭俊浩 1杜光 1王萍韬1
作者信息
- 1. 华中科技大学同济医学院附属同济医院药学部,武汉430030
- 折叠
摘要
Abstract
Objective To establish a high performance liquid chromatography ( HPLC) method for determination of berberine hydrochloride in zuning granules. Methods The HPLC method was employed to determine berberine hydrochloride. The separation was achieved on a Dionex C18 column (4.6 mm x 250 mm, 5 |xm) at a flow rate of 1.0 mL · min-1. The mobile phase is acetonitrile-0. 1% H3PO4(50:50). The detection wavelength was 265 nm, and the column temperature was set at room temperature. Results The linear range of berberine hydrochloride was within 5-50μg (r = 0.999 9). The average recovery was 100. 201% and RSD was 0. 49% . Conclusion The method is simple, accurate and repeatable. It is proved to be suitable for the quality control of zuning granules.关键词
小檗碱,盐酸/足宁颗粒/色谱法,高效液相Key words
Berberine hydrochloride/ Zuning granules/ High performance liquid chromatography分类
医药卫生引用本文复制引用
郭俊浩,杜光,王萍韬..高效液相色谱法测定足宁颗粒中盐酸小檗碱含量[J].医药导报,2012,31(8):1057-1058,2.
