湖北医药学院学报2012，Vol.31Issue(3)：201-203,208,4.
电喷雾离子阱质谱检测升麻素苷的正离子裂解途径
Fragmentation Pathways of Prim-O-glucosylcimifugin Revealed by Ion Trap Mass Spectrometry in Positive Mode
摘要
Abstract
Objective The structure and fragmentation pathway of prim-O-glucosylcimifugin were elucidated by electron spray ionization-ion trap mass spectrometry (ESI-MS). Methods A syringe pump was used for the direct loop injections of reference compound. The ESI source was used and operated in negative ion mode, and the flow rate was set at 0.3 mL/h. Typical operating conditions were described as follows: drying gas (N2) temperature of 350?, 10 L/min drying gas flow, 40 psi nebulizer gas (N2) pressure, and 4,500 V of capillary voltage. The full-scan MS spectra were obtained by scanning from m/z 100 -800. Results Quasi-molecular ion peak m/z 469 [M + H] + and the fragment ion of m/z 397 and 307 were detected by ESI-MS and ESI-MS2, respectively, and the main fragment ions of m/z 365,289,259,235,211,219,205 and 177 were found by ESI-MS3. The molecular ion fragment m/z 469 was stable and the major product ion at m/z 307 belongs to dihydrofurochromone. The characteristics of the fragment routes were discussed on the basis of ESI mass spectra. Conclusion It is suitable for determination the fragmentation and investigation of the fragmentation pathways of prim-O-glucosylcimifugin by ESI-MS in positive mode. The method is rapid and simple; it can provide the experimental data for studying pharmacokinetics in vivo and modifying structure for prim-O-glucosylcimifugin.关键词
升麻素苷/电喷雾离子阱质谱/裂解途径Key words
Prim-O-glucosylcimifugin/ Electron spray ionization-ion trap mass spectrometry/ Fragmentation pathway分类
医药卫生引用本文复制引用
陈琴华,李鹏,朱军..电喷雾离子阱质谱检测升麻素苷的正离子裂解途径[J].湖北医药学院学报,2012,31(3):201-203,208,4.基金项目
湖北省教育厅科研研究计划项目(D20122401和B20122424) （D20122401和B20122424）
