针刺研究2012，Vol.37Issue(4)：302-307,6.
针刺不同腧穴对失眠大鼠下丘脑γ-氨基丁酸和γ-氨基丁酸A受体的影响
Effect of Acupuncture at Different Acupoints on Expression of Hypothalamic GABA and GABAA Receptor Proteins in Insomnia Rats
摘要
Abstract
Objective To observe the effect of acupuncture of "Shenmai" (BL 62) and "Zhaohai" (Kl 6) ,"Shenmen" (HT7), etc. on the expression of hypothalamic y-aminobutyric acid (GABA) and GABAA receptor (GABAAR) proteins in experimental insomnia rats so as to explore its mechanism underlying improving sleeping. Methods Seventy Wistar rats were randomly divided into normal control, model, "Sanyinjiao" (SP6), "Neiguan" (PC6), "Zusanli" (ST36), "Shenmen" (HT7),and "Shenmai" (BL62)-Zhaohai (KI6, BL62-KI6) groups, with 10 rats in each group. Insomnia model was established by intrape-ritoneal injection of chlorophenylalanine solution (PCPA, 1 mL/100 g). An acupuncture needle was inserted into each of the bilateral HT7, PC 6, SP6, ST36 and BL 62-Kf 6 respectively and manipulated for about 1 min, once daily for 7 days. Hypothamic GABA and GABAAR protein expressions were detected by immunohistochemistry. The animals' physical ability was evaluated by using pole-climbing test in a water tank. Results In comparison with the normal control group, the numbers of hypothalamic GABA immunoreaction (IR)- and GABAAR IR-positive neurons and the pole-climbing time were reduced significantly in the model group (P<0.05). While in comparison with the model group, the numbers of hypothalamic GABA IR-positive neurons and those of hypothalamic GABAAR IR-positive neurons in the HT7, PC6, SP6, ST36 and BL62-KI 6 groups, as well as the pole-climbing duration in the SP 6, ST 36 and BL 62-KI 6 groups were increased considerably (P<0. 05, P<0. 01). The effects of HT 7 and BL 62-KI 6 groups were significantly superior to those of PC 6, ST 36 and SP 6 groups in up-regulating GABA and GABAAR expression , and the effect of BL 62-KI 6 group was remarkably better than those of HT 7, PC 6, SP 6 and ST 36 groups in lengthening the pole-climbing time (P<0. 05). Conclusion Acupuncture can effectively suppress insomnia induced down-regulation of hypothalamic GABA and GABAAR in rats and lengthen pole-climbing time, which may contribute to its effect in relieving insomnia.关键词
针刺/失眠/下丘脑/γ-氨基丁酸 γ-氨基丁酸A受体Key words
Acupuncture/ Insomnia/ Hypothalamus/ y-aminobutyric acid/ GABAA receptor分类
医药卫生引用本文复制引用
周艳丽,高希言,王培育,任珊..针刺不同腧穴对失眠大鼠下丘脑γ-氨基丁酸和γ-氨基丁酸A受体的影响[J].针刺研究,2012,37(4):302-307,6.基金项目
国家自然科学基金面上项目(81173335)、河南中医学院博士科研基金(BSJJ 2009-39) （81173335）
