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PHGPx在体外共培养山羊睾丸生精细胞中的定位研究

施力光 荀文娟 岳文斌 张春香 周汉林 侯冠彧 杨茹洁

中国草食动物科学2012,Vol.32Issue(4):19-21,3.
中国草食动物科学2012,Vol.32Issue(4):19-21,3.

PHGPx在体外共培养山羊睾丸生精细胞中的定位研究

A Study on Localization of Goat PHGPx in the Co-culture Spermatogenic Cells in Vitro

施力光 1荀文娟 2岳文斌 2张春香 2周汉林 2侯冠彧 1杨茹洁1

作者信息

  • 1. 中国热带农业科学院热带作物品种资源研究所,海南儋州 571737
  • 2. 山西农业大学动物科技学院,太谷030801
  • 折叠

摘要

Abstract

The objective of this study was to observe the expression of PHGPx in the co-culture spermatogenic cells in vitro. On the basis of the spermatogenic cells and sertoli cells co -cultured system, the slides of cells were stained for detecting the expression of PHGPx by using immunocytochemistry method. The results showed that PHGPx was mainly expressed in the cytoplasm of spermatids, but no expression in spermatogenium or sertoli cells. It suggested that PHGPx should play special roles in regulating the differentiation of spermatids during spermatogenesis, but the mechanism needed further research.

关键词

磷脂氢谷胱甘肽过氧化物酶/生精细胞体外共培养/山羊睾丸/细胞免疫化学

Key words

PHGPx/co-cultured spermatogenic cells in vitro/goat testicles/immunocytochemistry

分类

农业科技

引用本文复制引用

施力光,荀文娟,岳文斌,张春香,周汉林,侯冠彧,杨茹洁..PHGPx在体外共培养山羊睾丸生精细胞中的定位研究[J].中国草食动物科学,2012,32(4):19-21,3.

基金项目

中央级公益性科研院所基本科研业务费专项资金(1630032012022) (1630032012022)

中国草食动物科学

2095-3887

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