中国草食动物科学2012,Vol.32Issue(4):19-21,3.
PHGPx在体外共培养山羊睾丸生精细胞中的定位研究
A Study on Localization of Goat PHGPx in the Co-culture Spermatogenic Cells in Vitro
摘要
Abstract
The objective of this study was to observe the expression of PHGPx in the co-culture spermatogenic cells in vitro. On the basis of the spermatogenic cells and sertoli cells co -cultured system, the slides of cells were stained for detecting the expression of PHGPx by using immunocytochemistry method. The results showed that PHGPx was mainly expressed in the cytoplasm of spermatids, but no expression in spermatogenium or sertoli cells. It suggested that PHGPx should play special roles in regulating the differentiation of spermatids during spermatogenesis, but the mechanism needed further research.关键词
磷脂氢谷胱甘肽过氧化物酶/生精细胞体外共培养/山羊睾丸/细胞免疫化学Key words
PHGPx/co-cultured spermatogenic cells in vitro/goat testicles/immunocytochemistry分类
农业科技引用本文复制引用
施力光,荀文娟,岳文斌,张春香,周汉林,侯冠彧,杨茹洁..PHGPx在体外共培养山羊睾丸生精细胞中的定位研究[J].中国草食动物科学,2012,32(4):19-21,3.基金项目
中央级公益性科研院所基本科研业务费专项资金(1630032012022) (1630032012022)