摘要
Abstract
Newborn calves as experimental subjects, preparation and culture were done after cryopreservation of newborn bovine sertoli cells,and characteristics of sertoli cells were observed.The results showed that newborn calf lestis seminiferous tubules were solid tissue, spermatogonial stem cells were arranged in the seminiferous tubules of the central or sub-central. Sertoli cells packed ,and attached to the base membrane. The number of effective cells through digestion of testicular and isolated seminiferous tubules were more than that in the scissors method. Sertoli cells in vitro,4 h after adherent ,3~4 d covered dish bottom wall,and passage cells growed rapidly,proliferation of generation twice a day. HE staining,cytoplasmic staining lighter and darker nuclei,were round or oval central or deviation in the cytoplasm,prominent nucleoli. After cryopreservation, the recovery rate was 65 % or more. Sertoli cells after thawing in vitro, adherent cells began at 4 h, the number of adherent cells increased significantly at 6 h, the majority of the cells were adherent 12 h later and covered dish bottom wall in 3~4 days. 关键词
犊牛/睾丸支持细胞/体外培养/冷冻保存Key words
newborn bovine/sertoli cells/ in vitro culture/cryopreservation
分类
农业科技
