中国动脉硬化杂志2012，Vol.20Issue(5)：440-444,5.
吡格列酮对糖尿病大鼠血管钙化的影响及机制
The Effect and Mechanism of Pioglitazone on Vascular Calcification in Diabetic Rats
车星星 1边云飞 1卫娜 1王泽慧 1肖传实1
作者信息
- 1. 山西医科大学第二医院,山西省太原市 030001
- 折叠
摘要
Abstract
Aim To explore the effect and mechanism of pioglitazone on vascular calcification (VC) in diabetic rats. Methods Rat model of vascular calcification and diabetes (DM) was established by injection of vitamin D3 plus warfarin and streptozocin( STZ). Thirty six ten-week-old male SD rats were randomely divided into six groups; control group, DM group, VC group, VC + DM group, VC + PIO group, VC + DM + PIO group. Calcification was confirmed by Von Kossa staining, and calcium content and alkaline phosphatase (ALP) activity of the vascular tissue were measured. The expression of osleoprotegerin (OPG) mRNA and protein were examined by qRT-PCR and immunohistochemistry. Results Massive black granules were observed in the vascular smooth muscle cells and interstitial tissue by Von Kossa staining in VC group and VC + DM group. Calcium content and ALP activity of VC + DM group were 3. 63 times and 1. 35 times higher than DM group, and 3.69 times and I. 30 times(P<0. OS)higher than VC group, but the expression of OPG mRNA and protein were reduced( P < 0.05). Calcium content and ALP activity of VC + DM + PIO group decreased 13.70% and 18.04% than VC + DM group(P <0. 05), but the expression of OPG mRNA and protein increased(P< 0. 05). Conclusion Pioglitazone can reduce the degree of vascular calcification and increase the expression of OPG mRNA and protein. OPG is likely to be one of the main factors of inhibiting vascular calcification.关键词
血管钙化/糖尿病/吡格列酮/骨保护素Key words
Vascular Calcification/Diabetes/Pioglitazone/Osteogrotegerin分类
医药卫生引用本文复制引用
车星星,边云飞,卫娜,王泽慧,肖传实..吡格列酮对糖尿病大鼠血管钙化的影响及机制[J].中国动脉硬化杂志,2012,20(5):440-444,5.
