中国动脉硬化杂志2012，Vol.20Issue(5)：445-450,6.
人剪切修复基因着色性干皮病基因D对人脐静脉内皮细胞的促凋亡作用
The Promoting Effects of Xeroderma Pigmentosum D Gene on Proliferation of Human Umbilical Vein Endothelia Cells
张南 1李菊香 1丁浩 1洪葵 1吴清华 1程晓曙1
作者信息
- 1. 南昌大学第二附属医院心内科江西省分子医学重点实验室,江西省南昌市 330006
- 折叠
摘要
Abstract
Aim To investigate pro-apoptosis effects of xeroderma pigmentosum D( XPD) gene on human umbilical vein endothelia cells ( HUVEC). Methods Recombinant plasmid pEGFP-N2/XPD and vacant vector plasmid pEGFP-N2 were transient transfected into HUVEC by lipoaome 2000 method,with the same genetic background and algebra HUVEC as blank controls. The experiments were divided into three groups; control group, pEGFP-N2 group and pEGFP-N2/XPD group. The expression of green fluorescent protein was observed through fluorescence microscopy; the cell apop-losis rate was examined by flow cylometry; through RT-PCR and Western Blot, the expression levels of XPD,Bcl-2 and Bax and wt-p53 were detected jthe cell growth was detected by MTT. Results Green fluorescences were observed in the cells transfected with pEGFP-N2/XPD or pEGFP-N2, indicating that the plasmids were transfected successfully. Flow cy-tometry results showed that overexpression of XPD increased the apoptosic rate of HUVEC (P < 0.05 or P < 0. 01). RT-PCR results and Western Blot results showed that the transfection of pEGFP-N2/XPD increased the expression of XPD,Bax and wt-p53 (P < 0. 05), decreased the expression of Bcl-2 (P < 0.05); MTT results showed thai the transfection of pEG-FP-N2/XPD inhibited the cell growth( P <0. 05). Conclusions XPD gene can promote HUVEC apoptosis. Therefore , down-regulaling the expression of XPD gene is likely to be potential molecular target for treatment of atherosclerosis.关键词
剪切修复基因XPD/人脐静脉内皮细胞/细胞凋亡Key words
Xeroderma Pigmentosum Dj Human Umbilical Vein Endothelial Cell/Cell Apoptoaia分类
医药卫生引用本文复制引用
张南,李菊香,丁浩,洪葵,吴清华,程晓曙..人剪切修复基因着色性干皮病基因D对人脐静脉内皮细胞的促凋亡作用[J].中国动脉硬化杂志,2012,20(5):445-450,6.
