中国动脉硬化杂志2012，Vol.20Issue(7)：625-630,6.
肿瘤坏死因子受体2基因多态性与冠心病分型的相关性分析
Association of Tumor Necrosis Factor Receptor 2 Polymorphism and Clinical Classification with Coronary Heart Disease
摘要
Abstract
Aim To investigate the relationship between the single nucleotide polymorphism (SNP) of tumor necrosis factor receptor 2 (TNFR2) gene and soluble TNFR2 (sTNFR2) with coronary heart disease (CHD) in Shanxi population. Methods Two hundred and fifty CHD patients confirmed by coronary angiography (CAG) were enrolled, 98 healthy subjects served as control group. CHD group was divided into stable angina pectoris (SA, n =54), unstable angina pectoris (UA, n = 110) and acute myocardial infarction (AMI, n = 86) according to the clinical symptom. The clinical information about disease history, physical examination, assistant examination and CAC of all patients were recorded. The polymorphism of TNFR2 gene ( +676) was detected by the polymerase chain reaction-ligase detection reaction (PCR-LDR) and ihe concentration of sTNFR2 was detected by enzyme-linked immunosorbent assay (EUSA). The association of TNFR2 gene polymorphism in different clinical situation with the level of sTNFR2 were analysed. Results -(1) On 676 site, there were three genotypes: TT, TG and GG. (2 ) The frequency of GG type and G allele were significantly different between CHD and control (P<0.05). (3)Compared with UA and AMI group, ihe frequency of T/G allele was decreased in SA group (P < 0.05), but no difference was found between UA and AMI group. (4) The levels of sTNFR2 were signifieantly higher in CHD patients than controls (P<0. 05) ; In three genetypes of CHD patients the levels of sTNFR2 were increased compared with control group, but in both CHD group and control group the relation betwean levels of sTNFR2 and TG genotype and G allele were not found. (5)Although no differences were found in blood glucose, total cholesterol and systolic blood pressure between TG + GG type and TT type, the risk of patients with TG + GG type suffer from CHD was 1.648 times of those patients with TT type. Conclusions The TG + GG genetype confers independent risk factor of CHD in Han nationality population in Shanxi Province. Levels of sTNFR2 can reflect the body' s inflammatory state for monitoring CHD. There is no relationship between level of &TNFR2 and genetype.关键词
肿瘤坏死因子受体2基因/基因多态性/冠心病Key words
Tumor Necrosis Factor Receptor 2 Gene/Single Nucleotide Polymorphisms/Coronary Heart Disease分类
医药卫生引用本文复制引用
卫娜,李俊男,任俊峰,肖传实,边云飞..肿瘤坏死因子受体2基因多态性与冠心病分型的相关性分析[J].中国动脉硬化杂志,2012,20(7):625-630,6.基金项目
山西省科技攻关项目(20090311057-4) （20090311057-4）
