中国肺癌杂志2012，Vol.15Issue(7)：391-398,8.DOI:10.3779/j.issn.1009-3419.2012.07.01
吲哚-3-甲醇对肺癌细胞放射敏感性的EGFR依赖性调节
EGFR-dependent Impact of Indol-3-Carbinol on Radiosensitivity of Lung Cancer Cells
摘要
Abstract
Background and objective Indole-3-carbinol (I3C) is a naturally occurring phytochemical found in cruciferous vegetables. The aim of the present study is to investigate the influence of 13C on radiosensitivity in epidermal growth factor receptor (EGFR)-positive and EGFR-negative lung cancer cell lines. Methods Human lung adenocard-noma NIH-H197S cells and human lung squamous carcinoma NIH-H226 and NIH-H520 cells were routinely cultured in RPMI-1640. MTT assay and donogenic assay were used to detect cell growth and survival, respectively. Western blot and RT-PRC assay was employed to detect EGFR protein and mRNA expression. Results 5 祄ol/L of I3C significantly reduced radiosensitivity of EGFR-positive NIH-H197S and NIH-H226 cells, but failed to affect radiosensitivity of EGFR-negative NIH-HS20 cells. Furthermore, I3C caused an increased expression of total EGFR and pEGFR (Y84S) protein in NIH-H197S and NIH-H226 cell lines, but not in NIH-HS20 cell line. A reduction of EGFR expression by EGFR-siRNA significantly inhibited I3C-caused radioresistance in NIH-H1975 cells. Conclusion Our data presented here for the first time demonstrate that I3C reduces radiosensitivity of lung cancer cells by mediating EGFR expression, indicating that EGFR may be an important target for I3C-mediated radioresistance in lung cancer.关键词
吲哚-3-甲醇/肺肿瘤/放射敏感性/表皮生长因子受体Key words
Indol-3-carbinol/ Lung neoplasms/ Radiosensitivity/ Epidermal growth factor receptor分类
医药卫生引用本文复制引用
肖骁,孟庆慧,徐加英,焦旸,Eliot M Rosen,樊赛军..吲哚-3-甲醇对肺癌细胞放射敏感性的EGFR依赖性调节[J].中国肺癌杂志,2012,15(7):391-398,8.基金项目
本研究受国家自然科学基金面上项目(No.81071906,No.81172127)和江苏省高等学校优秀科技创新团队(No.SZ132901)资助 （No.81071906,No.81172127）
