中国感染控制杂志2012，Vol.11Issue(2)：81-85,5.
ATP生物荧光检测技术相关性基础研究
Association study on adenosine triphosphate biofluorescence detection technology
易滨 1刘军 1王芳 1涂显春 1赵晓晓 1赵江丽1
作者信息
- 1. 中国人民解放军第309医院,北京100091
- 折叠
摘要
Abstract
Objective To test the relation between adenosine triphosphate (ATP) biofluorescence detection technology and bacterial colony forming unit (CFU) and blood content Methods Escherichia coli (E. coli) ATCC 8099 and heathy controls' blood were diluted to the concentration of 10"' ,10-2,10-3,10-4,10-5,10-6 and 10-7', respectively, then lysate, luciferase and ATP standard substance were added, relative light unit (RLU) values were determined twice by fluorimeter, ATP contents(amol) were converted according to formulaCATP content = A1/A2-A1 × 106); hemoglobin values were measured by hematology analyzer to reflect protein residue leveL Curve demarcate standardization was made to show the relation between diluted bacteria, hemoglobin content and ATP content. Results Bacterial CFU and ATP content logarithm values (y = 1. 07x - 0. 55, R2 = 0. 99), bacterial CFU and ATP RLU logarithm values (y=1.14x + 0. 33,R2 =0. 99) showed linear relationship respectively. There was no significant difference between the logical values figured out by different RLU values and the actual values. After hemodilution, hemoglobin and ATP RLU logarithm values also showed linear relationship (y= 1. 03x-8. 42, R2 =0. 99). Conclusion ATP biofluorescence detection technology can detect the content of bacteria and protein through ATP value and RLU, it can determine contamination degree and clean effect of medical equipments and object surface, it's a new, sensitive and rapid detection method.
关键词
ATP/生物荧光/ATP生物荧光检测/清洗/感染控制Key words
ATP/biofluorescence/ATP biofluorescence detection/ clean/infection control分类
医药卫生引用本文复制引用
易滨,刘军,王芳,涂显春,赵晓晓,赵江丽..ATP生物荧光检测技术相关性基础研究[J].中国感染控制杂志,2012,11(2):81-85,5.
