中国骨伤2012，Vol.25Issue(7)：587-591,5.DOI:10.3969/j.issn.1003-0034.2012.07.014
适宜电刺激对成骨细胞平面培养下细胞增殖分化及BMP-2表达的实验研究
Proliferation,differentiation and BMP-2 expression of osteoblasts cultured in the plane under suitable electrical stimulation
摘要
Abstract
Objective :To observe proliferation and differentiation of osteoblasts cultured in the plane on appropriate electrical stimulation,to specify whether it promote the proliferation,and observe expression of BMP-2 on electrical stimulation. Methods: Osteoblasts were extracted from the skull of rabbit offspring and cultured. Cells after the 2nd generations were cultured. In experimental group,cells had electrical stimulation,and same stimulation time and intensity were given. In control group cells had not electrical stimulation. The proliferation and differentiation were detected at different time,and BMP-2 protein expression was analyzed. Results: The cell morphology of experimental group in 8 days under the light microscope was observed and showed a lot of proliferation of osteoblasts, pleumorphic changes, in 6 to 8 days a small amount of Calcified spots was also observed;while in the control group,proliferation was slower. Differentiation of the experimental group was significantly,alkaline phosphatase staining and calcium nodules were positive,quantitative analysis of alkaline phosphatase in-creaseed significantly. Ex|ierimental group showed that BMP-2 was gradually increased by immunohistochemistry analysis. Conclusion: Electrical stimulation can promote the proliferation and differentiation of osteoblasts and achieved the increase-ment the number of cells in short-term, intracellular staining by immunohistochemistry showed the increasement in expression of BMP-2.关键词
成骨细胞/电刺激/细胞增殖/骨基质形成蛋白(BMP-2)/动物实验Key words
Osteoblasts/Electric stimulation/Cell proliferation/Bone morphogenetic protein (BMP-2)/Animal experimentation引用本文复制引用
王伟,李秀翠,程少文,陈庆玉,彭磊,林忠勤,张伟,寇冬权..适宜电刺激对成骨细胞平面培养下细胞增殖分化及BMP-2表达的实验研究[J].中国骨伤,2012,25(7):587-591,5.基金项目
浙江省科技厅大学生孵化项目研究内容(编号:2010R413060) （编号:2010R413060）
