中国临床药理学与治疗学2012，Vol.17Issue(7)：750-754,5.
黄芩素对星形细胞瘤SHG-44细胞增殖、侵袭迁移的影响
Influence on cell proliferation and invasiveness of human astrocytic glioma cell line SHG-44 by baicalein
摘要
Abstract
AIM: To investigate the influence on cell proliferation, apoptosis, cell cycle and cell invasiveness after human astrocytic glioma cell line SHG-44 were treated by baicalein with the valious concentrations. METHODS: Human astrocytic glioma cell line SHG-44 were cultured in vitro and treated in various concentrations of baicalein, then MTT method, flow cytometer (FCM) analysis, transwell assay and Western Blot were applied to measure cell growth, cell cycle and cell invasiveness. RESULTS: Bacalein treatment inhibited proliferation of SHG-44 cells in a time and dose-dependent manner. Baicalein treatment induced increasing apoptosis in a dose-dependent manner. Compared with the control, Baicalein caused a significant G,./Gi phase arrest (P<0.05) and a decrease in the percentage of cells in S-phase by approximately 18% ( P <C 0. 05) in a dose-dependent manner. No significant change for G2/M phase cells was observed. The result of Transwell assay exhibited that cell number through the artificial basal membrane was obviously less when compared with the control after 48 hours (P<0. 05). CONCLUSION: Bacalein treatment inhibited growth of SHG-44 cells in a time and dose-dependent manner. Baicalein can inhibit cell invasion and migration of human astrocytic glioma cell line SHG-44. Bacalein may be a useful therapeutic strategy for hum astrocytic glioma.关键词
黄芩素/SHG-44细胞/细胞增殖/细胞周期/侵袭迁移Key words
Baicalein/SHG-44 cell/ Proliferation/ Cell cycle/ Invasiveness分类
医药卫生引用本文复制引用
毛捷,盛莉莉,徐善水,王宣之..黄芩素对星形细胞瘤SHG-44细胞增殖、侵袭迁移的影响[J].中国临床药理学与治疗学,2012,17(7):750-754,5.基金项目
安徽省中医药研究课题项目资助(2009ZY40) （2009ZY40）
