中国临床药理学与治疗学2012，Vol.17Issue(7)：779-784,6.
焦磷酸测序分析ABCG2基因多态性方法的建立
Development of pyrosequencing method for detection of ABCG2 polymorphisms
摘要
Abstract
AIM: To establish a pyrosequencing based method for detection ABCG2 34G>A and 421OA polymorphisms and to determine the frequency of these polymorphisms in healthy Chinese. METHODS: After preparation of gD-NA from blood of 200 subjects, the target fragments were amplified by PCR, polymorphisms were detected on PyroMark ID by pyrosequencing technology. The reliability of pyrosequencing methods were validated by repeat tests and Sanger sequencing. RESULTS: We established a new pyrosequencing method to detect the AB-CG2 34G>A and 421OA polymorphisms polymorphisms in healthy Chinese. The detection rate and repetition rate were both 100%. The frequencies of ABCG2 34G and 34 A alleles were 79. 5% and 20. 5% , respectively. The allele frequencies of ABCG2 421C and 421A were 72. 7% and 27. 8%, respectively. Genotype frequencies match the Hardy-Weinberg equilibrium. CONCLUSION: These pyrosequencing assays to detect ABCG2 polymorphisms are proved to be a rapid, accurate and high-throughput alternative to conventional methods, and it can be a preferred option in research and clinical application.关键词
药理学/焦磷酸测序/ABCG2/乳腺癌耐药蛋白/单核苷酸多态性Key words
Pharmacology/ Pyrosequencing/ABCG2/ BCRP/ SNPs分类
医药卫生引用本文复制引用
万子睿,谢海棠,俞竞,刘英姿,曾飞跃,王果..焦磷酸测序分析ABCG2基因多态性方法的建立[J].中国临床药理学与治疗学,2012,17(7):779-784,6.基金项目
国家自然科学基金项目(81072706,81173134) （81072706,81173134）
高等学校博士学科点专项科研基金资助课题(200901621210024) （200901621210024）
湖南省科技计划项目(2009JT3020) （2009JT3020）
中央高校基本科研业务费(2012QNZT085,2012QNZT133) （2012QNZT085,2012QNZT133）
