中国临床医学2012，Vol.19Issue(3)：193-197,5.
二维法和三维法诱导小鼠胚胎干细胞定向分化为内皮细胞
Differentiation of Murine Embryonic Stem Cells into Endothelial Cells Using Two-Dimensional Method and Three-Dimensional Method
摘要
Abstract
Objective:To compare the efficiency of two-dimensional (2D) method and three-dimensional (3D) method in the induction of differentiation of murine embryonic stem cells (ESCs) toward endothelial cells (ECs). Methods: Both 2D method and 3D method were used to induce the differentiation of murine ESCs toward ECs. Supplementation of vascular endothelial growth factor (VEGF) and isolation of fetal liver kinase 1 positive (Flk-1 + ) cells were done for further purification. Biological characteristics of embryonic stem cell-derived endothelial cells(ESDECs) were confirmed by reverse transcription-polymerase chain reaction (RT-PCR),immunofluorescence test,DiI-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake assay. Re-sultS: The proportion of Flk-1 + cells acquired using 2D method was significanly higher than using 3D method (P<0. 05). In the 2D culture system,Flk-1 + cells exhibited the characteristics of endothelial-like cells with cobblestone morphology and striated smooth muscle-like cells. After further purification by manual selection,those cells displayed the morphology of endothelial cells with high purity and they were able to be subcultured. The ESDECs acquired through both methods expressed endothelial cell-specific markers,formed tube-like channels and incorporated Dil-Ac-LDL. Conclusions: Both 2D method and 3D method can be used to induce the differentiation of murine ESCs into ECs in vitro,but the former is more efficient than the latter. Supplementation of vascular VEGF and isolation of Flk-1 + cells can help to improve the purification of ESDECs.关键词
胚胎干细胞/内皮细胞/细胞分化Key words
Embryonic stem cell/Endothelial cell/Cell differentiation分类
医药卫生引用本文复制引用
高斌,符伟国,竺挺,张祥满,董智慧,王玉琦..二维法和三维法诱导小鼠胚胎干细胞定向分化为内皮细胞[J].中国临床医学,2012,19(3):193-197,5.基金项目
国家自然科学基金资助项目(编号:30571821) （编号:30571821）
